Application Compendium for DNA, RNA, Protein and Cell Analysis [antikvár]

I. Cell fluorescence analysisProtein expression monitoring Cell surface antibody staining - CD4 in CCRF CEM T-cells Cell surface antibody staining - CD3 in T-cell leukemia CDS expression in T-cell leukemia via on-chip staining Intracellular glucocorticoide receptor (GR) antibody staining in H4 hepatocytes Analyzing a limited number of cells Baculovirus titre determination Upregulated gene expression in primary cellsTransfection efficiency monitoring Green fluorescent protein in CHO cellsOn-chip staining of GFP expression for optimizing transfection conditions with different DNAilipid ratiosVerification of stable transfected cell clones by on-chip antibody staining Transfection of primary cellsApoptosis detection Detection of phosphatidylserine on the cell surface via Annexin V binding Intracellular Caspase-3 antibody staining assayFast Annexin protocol for time course of apoptosis induction via anti-FAS antibodyApoptosis detection in primary cellsGene silencing in cell culture siRNA transfection optimization Monitoring of gene silencing experimentsCells - othersIdentification and counting of blood cells in whole blood samples