Cell Culture [antikvár]

PrefaceMany of the problems that have caught the interest and imagination of biochemists are studied best with cultured cells. We oflfer in this volume, in a format familiar to investigators in biochemistry, the general techniques necessary for working with cells in culture and illustrate such general methods with specific examples from the large variety of cells that have been cultivated.The tools and methods for ceU culture are presented in Part I. Part II provides a group of specialized techniques that are useful for many of the applications that biochemists and other investigators with their widely different approaches may require. Part III is concerned with specific methods for specific ceU types that have been chosen to represent the wide range of cells that may now be prepared.There is some duplication in the presentations. For example, portions of certain methods are repeated in one or another form both in Part I and Part III. We believe that this repetition is necessary to convey faithfuUy to the reader a complete method of proven effectiveness. Additionally, we hope that a heuristic effect will be achieved that will enable investigators unfamiliar with ceU culture to assess what is available and to predict what might be most suitable for their own purposes.William B. Jakoby Ira H. Pastan