Molecular Cell Biology Laboratory Manual For Students Of Dentistry [antikvár]

PREFACE The development of molecular cell biological sciences has dramatically sped up in the last few decades. Attention has focused on new techniques that are indispensable for the everyday medical practice and research. The present edition of this laboratory manual is based on previous handouts edited by József Szeberényi and László Komáromy, but it has been modernized and some of the practicals have been rewritten. In the Molecular Cell Biology Laboratory Manual for Students of Dentistry there are practices, to be performed in the second semesters of the compulsory course Molecular Cell Biology, for Students of Dentistry. At the beginning of each chapter the theoretical background necessary to understand the practical part is summarized, and followed by the description of the experiments. The aim of molecular cell biology practices is to make the learning of the simplest experimental methods for the students possible. More complex experiments and the ones the duration of which would exceed the length of the lab time, are presented in form of demonstrations for the students. The experiments can only be performed and understood during the available lab time, if the students come prepared for the practices. It is therefore very important that prior to practices students read the theoretical and practical parts of the appropriate chapter. The laboratory manual also serves as a workbook, students need to draw or write the results of the experiments into the empty spaces provided after the description of each experiment. We thank Prof. József Szeberényi, from whom we have received a lot of useful advice and professional help during the planning of the experiments and preparation of the text. Prof. László Komáromy's experience and practical approach has also greatly contributed to the preparation of the laboratory manual. We thank Hajnalka Ábrahám, András Balogh, Alexandra Harci, Ágnes Kemény, Mária Németh and Judit Varga for the thorough proofreading of the text. Some of the slides presented during practices were prepared in the Department of Medical Biology, while others were obtained from various institutes of the University of Pécs. We are indebted for the sections to Hajnalka Ábrahám (Central Electron Microscope Laboratory) who provided the [3H]-thymidine, bromodeoxyuridine sections, the immune histochemical sections detecting vimentin, glial fibrillary acidic protein and neurofilaments; Anikó Perkecz (Department of Pharmacology and Pharmacotherapy), who gave a lot of useful professional advice during the preparation of the immunohistochemical slides; Zoltán Szereday,