Proceedings of the 16th International Symposium on Analytical and Environmental Problems - CD-vel [antikvár]

ABSTRACT (Aonrimc hisDorus) and four different A. From^he íhiitbodies the phenol content was measured by spectrophotometer and expressed .n gS acid equivalent, while totál antioxidant capacity of extracts was determined by the FRAP method and expressed in ascorbic acid equivalent. Our results revealed a tight correspondence between the totál antioxidant capacity and totál phenol content of samples. We concluded that differences can be found between mushroom species, moreover between varieties in accumulation of secondary metabolites. The measured parameters differed in stipes and caps. Based on these results it is possible to selecM. subrufescens cultivars in other point of view, underline the fact that medicinái products and pharmaceuticals prepared from this mushroom species can be distinct. INTRODUCTION The diseases in humán body like cancer, cardiovascular defeat, immundiseases are often caused by presence of free radicants, that can be potential harmful for lipids, proteins and carbohydrates too. The free radicants may effect DNA as well. These free radicants appear in normál metabolism, but firstly environmental conditions and other biotic and abiotic stress factors increase their concentrations. In the past years instead of synthetic antioxidants (e.g. BHA, BHT, TBHQ) the researchers focused on natural antioxidant due to their effect of disease prevention. The low molecular weight antioxidants (Yitamin-A, D, flavonoids, glutation, etc.) are able to prevent different sickness. The mentioned compounds are present in food and usually get into humán body by feeding. These molecules reserve normál physiological functions and in many cases help in prevention of diseases related with oxidative damage. Numerous studies have shown an inverse relationship between fruit and vegetables consumption and the risk of developing several types of cancers, as well cardiovascular and other diseases (Ames et al., 1993; Weisburger, 1999). In the past decades many papers were published in nutritional effect and bioactive components of edible mushrooms. Fungi are able to accumulate secondary metabolites, phenohc compounds, polyketides, terpenes and steroids. As a research demonstrated (Ishikawa et al., 1984) the phenolic compounds of mushrooms could have antioxidant activity. The Basidiomycota fungus Agaricus subrufescens (Peck) (syn. A. blazei (MURRILL), syn. A. brasiliensis) is a well accepted medicinái mushroom around the world. Lot of reports available its medicinái effect in cancer therapy and its beta-glucan compounds are well characterized. In present research four different Agaricus subrufescens cultivars (marked: 853; 1105; Brazil; Si), white button mushroom (marked: White) and the cream type (marked: Brown) Agaricus