Recent Progress in Perinatal Medicine V. [antikvár]

STUDY OF THE UTERINE PEPTIDES IN THE DYNAMICS OF PREGNANCY AND LABOR P.Ya. Kintraya, I.D. MamamtavrishviU, D.l. Mikeladze Academician Chachava Scientific Research Institute of Perinatal Medicine, Obstetrics and Gynaecology, Tbilisi, GSSR Nowadays the immunochemical analysis of the peptides is the most widely — spread method of their quantitative value in the biological liquids and tissues. However, one should note that well-known radio-immunologic and immunoenzymatic methods which are based on the reaction of the antigen precipitation by the antibody are slightly specific relative to neuropeptides. This is caused by the fact that almost all the biologically active peptides have their protein-precursor which contains domains similar to the given peptide and amino acid sequences manifesting similar immune reactivity. That's why the definition of the peptide concentration in liquids and tissues by means of the precepitation of the antigens by the specific antibodies will not adequately reflect veritable content of the peptides in the studies objects. Thus, methods based on the immunochemical analysis enable to carry out the quantitative definition not the strictly definite peptide but set of these compounds including proteins-precursors which have the common antigenic determinant. Besides, these methods can examine only one or two-three definite componds. That's why they are applied only for screening of the definite substance. Methods based on the high-effective division of the peptides according to their molecular weight partition coefficients, ionized force, polarity and other physical and chemical criteria have no such fefects. The method of the high-performance liquid chromatography is considered to be one of such methods, it enables us to define and identify in the complex mixture of substances not only well-known peptide compounds but also reveal endogenous substances-regulators of the unknown nature according to the retention time. Besides, above mentioned method beneficially differes from those existed before as it enables us to carry out the simultaneous definition of the complete map of the peptides of the investigated tissue. Taking into consideration the literary data on the possible participation of the peptidergic neuron systems in the regulation of the labor act (1, 2, 3), we decided to study the complete peptide hypothalamic and uterine map in pregnant and non-pregnant animals through the method of the high-performance liquid chromatography. Puberal the inbred white rat female weight 200-250 g were used in the experiment. In labor after the birth of the first fetus rats were de-